左归降糖解郁方对糖尿病并发抑郁症大鼠海马神经元病理形态及凋亡的影响

左归降糖解郁方对糖尿病并发抑郁症大鼠海马神经元病理形态及凋亡的影响
作者：柳卓 孟盼 杨蕙
来源：《中国中医药信息杂志》2018年第09期

        摘要：目的 觀察左归降糖解郁方对糖尿病并发抑郁症（diabetes mellitus with depression，DD）大鼠海马神经元病理形态的影响，探讨其保护作用机制。方法 采用尾静脉注射链脲佐菌素联合慢性应激建立DD大鼠模型。72只SD大鼠随机分为正常组、模型组、阳性药组和左归降糖解郁方低、中、高剂量组，每组12只，分别给予相应药物。末次给药后，检测大鼠血糖及行为学变化，分别采用HE染色、尼氏染色、高尔基染色观察大鼠海马组织病理形态，免疫组化检测大鼠海马Caspase-3的表达。结果 与正常组比较，模型组大鼠血糖显著升高，海马区神经元数量显著减少，神经元凋亡增加，神经元胞体出现变形、树突棘减少等病理损伤情况；给予左归降糖解郁方干预后，模型大鼠高血糖和抑郁样状态缓解，海马神经元数量显著增加，神经元形态基本恢复正常，左归降糖解郁方高、中剂量组Caspase-3表达较模型组明显降低。结论 左归降糖解郁方可明显改善DD大鼠血糖和学习记忆能力，其机制可能与减缓海马损伤和凋亡、恢复神经元数量与形态有关。

        关键词：左归降糖解郁方；糖尿病并发抑郁症；海马神经元；病理形态；大鼠

        DOI：10.3969/j.issn.1005-5304.2018.09.011

        中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2018）09-0044-05

        Abstract： Objective To observe the effects of Zuogui Jiangtang Jieyu Prescription （ZGJTJYP） on pathological morphology of hippocampal neurons in rats of diabetes mellitus with depression （DD）； To discuss its protective mechanism of action. Methods DD rat model was established by tail vein injection of STZ combined with chronic stress. Totally seventy-two SPF rats were randomly divided into normal group， model group， positive medicine group， ZGJTJYP low-， medium-， and high-dose groups， with 12 cases in each group， and were given corresponding medicine. After the last administration， blood glucose and behavioral changes were measured in each group. HE staining， Nissl staining， and Golgi staining were used to observe the changes of pathological morphology in rat hippocampus. The expression of Caspase-3 in hippocampus was detected by immunohistochemistry. Results Compared with the normal group， blood glucose level was significantly increased in the model group； the number of neurons in the hippocampus decreased in the model group； the apoptosis increased in the model group； the neuronal soma showed deformation and reduced dendritic spines and other pathological damages in the model group. After intervention of ZGJTJYP， hyperglycemia and depression-like status of the model rats were relieved； the number of hippocampal neurons increased significantly； neuronal morphology basically returned to normal. The expressionof Caspase-3 in ZGJTJYP high- and medium-dose groups was significantly lower than that in model group. Conclusion ZGJTJYP can significantly improved blood glucose and learning and memory abilities in DD rats， which may be related to slowing hippocampal injury and apoptosis， and restoring neuronal quantity and morphology.

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