Hans Journal of Computational Biology 计算生物学, 2018, 8(2), 33-39Published Online June 2018 in Hans. http://www.hanspub.org/journal/hjcb https://doi.org/10.12677/hjcb.2018.82005文章引用: 李利斌, 孟昭娟, 王永强, 杨桂兰, 杨宗辉, 纪复勤, 韩梅香, 曹齐卫. 黄瓜抗坏血酸过氧化物酶基因的鉴定和特征分析[J]. 计算生物学, 2018, 8(2): 33-39. DOI: 10.12677/hjcb.2018.82005Identification and Characterization of APX Genes in CucumberLibin Li 1, Zhaojuan Meng 1, Yongqiang Wang 1, Guilan Yang 1, Zonghui Yang 1, Fuqin Ji 2, Meixiang Han 2, Qiwei Cao 1*1Key Laboratory of Greenhouse Vegetable’s Biology of the Shandong Province, Vegetable Science Observation and Experiment Station in Huang Huai District of Ministry of Agriculture (Shandong), Shandong Branch of National Improvement Center for Vegetables, Vegetable Institute of Shandong Academy of Agricultural Sciences, Jinan Shandong 2Wanfeng Seed Company Limited in Ningyang County, Shandong Province, Ningyang ShandongReceived: Jun. 27th , 2018; accepted: Jul. 12th , 2018; published: Jul. 19th , 2018 Abstract Plant ascorbate peroxidase (APX ) genes play significant roles in environmental stress response and development. However, the knowledge about them in the important vegetable cucumber is scarce. In this paper, 6 APX genes in cucumber genome were identified through bioinformatics method. And their chromosome location, gene exon-intron structure, phylogeny, and cis-elements were systematically analyzed. The results showed that cucumber APX genes distribute in different chromosomes, and their putative coding sequence size ranges from 750 bp to 1425 bp. They all have 8 or more exons, and their putative encoded proteins are predicted to localize in cytosol or chloroplast. There are typical ascorbate peroxidase domain, myristoylation sites, and phosphary-lation sites in APXs encoded proteins of cucumber. And also, multiple cis-elements responsive to different environmental stimuli and hormones are existed in cucumber APX upstream sequences, which suggests they may have some roles in environmental stress responses. KeywordsCucumis sativus L., Ascorbate Peroxidase Gene, Structure, Phylogeny, Cis-Element 黄瓜抗坏血酸过氧化物酶基因的鉴定和特征分析李利斌1，孟昭娟1，王永强1，杨桂兰1，杨宗辉1，纪复勤2，韩梅香2，曹齐卫1*1山东省农业科学院蔬菜花卉研究所，国家蔬菜改良中心山东分中心，农业部黄淮地区蔬菜科学观测实验*通讯作者。
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