2010,26(3):268~272神经解剖学杂志Chinese Journal of Neuroanato m y枸杞子提取液对单眼视觉剥夺性弱视大鼠视网膜的保护作用王文宏1,刘相和2,迟焕芳1*(1.青岛大学医学院病理学教研室,青岛266021;2.齐河县人民医院,德州251100)=摘要> 目的:观察枸杞子提取液(LBA)对视觉剥夺性弱视大鼠视网膜的保护作用,并探讨其可能的作用机制。方法:新生W i star大鼠21只,雌雄不限。随机分为空白对照组(5只)、治疗对照组(8只)及治疗组(8只)。1周后,缝合治疗对照组及治疗组大鼠单侧眼睑,建立动物模型。4周后,拆除手术缝线,并给予治疗组大鼠LB A 1m g/kg治疗,每日一次。空白对照组及治疗对照组自由进食。6周后处死所有大鼠,摘取眼球。应用伊红-苏木素(HE)染色观察视网膜的改变;应用TUNEL检测视网膜细胞凋亡的状况;应用免疫组织化学检测B c-l2蛋白(Bc-l2)、Bax蛋白(Bax)及caspase-3在视网膜细胞的表达。结果:与空白对照组相比,治疗对照组Bax及caspase-3的表达明显增强(P<0.05),Bc-l2的表达明显减弱(P<0.05);与治疗对照组比较,治疗组Bax及caspase-3的表达均明显减弱(P<0.05);而B c-l2的表达明显增强(P<0.05)。结论:LBA对视觉剥夺性弱视大鼠视网膜在视觉发育可塑期内可能具有保护作用,其机制可能与上调Bc-l2、下调Bax及抑制caspase-3的表达,减少细胞凋亡有关。=关键词> 弱视;枸杞子提取液;TUNEL;Bc-l2;Bax;大鼠Neuroprotective effects of Lyciu m barbaru m on t he retina ofm onocular vis ualdeprived a m blyopia in ratsWang W enhong1,L iu X ianghe2,ChiHuanfang1(1.Depart m ent of Pathol ogy,M edical S chool of Q i ngdao Un i versit y,Q i ngdao266021;2.J i he Peop le.s H ospita,l De i zhou,251100,Ch i na)=Abstract>Ob jective:T o observe the reti nal chang es of m onocu lar v isual depr i ved amb l yopia i n rat,and t o i nvesti ga te o f the neuropro tecti ve effect of the aqueous ex tract o f dried fruits o f Ly ci um barbaru m(LBA)on the rat and possi b le m echan is m.M e thods:A t o ta l of21newnata lW i star rats were random ly d i v i ded i nto3groups,blank contro l(BC,5 ra ts),trea t m en t contro l(TC,8ra ts)and treat m ent g roup(TG,8rats).A fter postnata l1w eek,t he m onocular palpebra w as se w ed i n TC and TG to m ake the ra tm ode.l A fter postna tal4w eeks,the su t ure w as dis mantled.TG sta rted to be fed 1mg/kg LBA da il y;both BC and TC we re fed no r m all y.A fter post nata l6w eeks,a ll the ratsw ere execu ted to re m ove se-lected eyeba ll s.T hen reti nal cells necrosis and apoptosis w as assessed by the me t hods o fHE sta i n i ng,TUN EL detection, t he expressi on of Bc-l2,Bax and caspase-3was detected by i m munohistoche m istry.R es u lts:T he positive expressi on o f Bax and caspase-3i n TC w as si gn ifi can tly h i gher t han that i n BC(P<0.05),and Bc-l2in TG w as si gn ifi cantly lowe r(P<0.05).Co m pared w ith TC,the positi ve expressi on of Bax and caspase-3w ere sign ifi cantl y lo w er i n TG(P<0.05),and t he pos i tive expression o f Bc-l2w as sign ifi cantl y h i ghe r in TG(P<0.05).Conc l u sion:LBA m i ght have neuropro-tecti ve e ffect on the reti na of monocu l a r v isual depr i ved a m blyopia i n ra t i n the cr itica l per i od o f v is ua l plastic ity dur i ng dev elopment,w hich m i ght be re lated w ith up-regu l a ting t he expressi on o f Bc-l2,neg ati ve regu lati ng the expression o f Bax,i nhi b iti ng the expression o f caspase-3and reduci ng ce ll u lar apoptos i s.=K ey word s> amb l yopia;LB A;TU NEL;Bc-l2;B ax;rat*通讯作者:迟焕芳电话:0532-********,E-m ai:l hfch i9200@163.co m
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