黑老虎中倍半萜类化合物的分离鉴定及抑制NO生成作用研究作者：石柳柳 李贺然来源：《中国医药导报》2016年第10期
[摘要] 目的 分离鉴定黑老虎中倍半萜类化合物，并考察化合物对一氧化氮（NO）释放的抑制作用。 方法 采用丙酮提取，半制备高效液相色谱等方法分离化学成分，波谱方法鉴定化合物的结构；脂多糖（LPS）和γ干扰素（IFN-γ）诱导的单核巨噬细胞RAW264.7作为细胞炎症模型，考察化合物对NO释放的抑制作用。 结果 分离得到了4个倍半萜类化合物，经波谱方法鉴定，分别为匙叶桉油烯醇（1）、（-）-7βH-柳杉二醇（2）、（-）-依兰油醇（3）、（+）-顺-菖蒲烯（4）。化合物1较强抑制LPS/INF-γ诱导NO的产生。 结论 化合物1～4均首次从南五味子属中分离得到，化合物1具有抗炎活性。
[关键词] 黑老虎；倍半萜；NO；抑制作用
[中图分类号] R927 [文献标识码] A [文章编号] 1673-7210（2016）04（a）-0027-04
Identification of Sesquiterpenes from Kadsura coccinea and reaserch on their Nitric Oxide inhibitory activities
SHI Liuliu LI Heran
College of Pharmacy， Soochow University， Jiangsu Province， Suzhou 215123， China
[Abstract] Objective To study the sesquiterpenes from kadsura coccinea and their nitric oxide （NO） inhibitory activities. Methods Acetone was used to extract， the compounds were isolated by semi-preparative HPLC method， their structures were identified by spectroscopy. The activated RAW 264.7 macrophages induced by LPS and IFN-γ were used to evaluate the inhibitory activities on NO production. Results Four sesquiterpenes were isolated and identified as ent-spathulenol， （-）-7βH-eudesmane-4α， 11-diol， （-）-T-muurolol， （+）-cis-Calamenene. Compound 1 had strong inhibitory activity on NO production. Conclusion Compound 1-4 are obtained from the Kadsura for the first time. Compound 1 has the anti-inflammatory effect.
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