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发布时间：2023-08-28 14:06:42 来源：文档文库

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通用引物是一般和载体多克隆位点两旁的序列匹配，或者是与载体里面的一些启动、终止元件匹配。这样不管载体插入什么DNA片段，都可以用通用引物扩出来。通用引物可以用来测序，但是只是"通用"，也不是万能的。引物名称3’AOX3'AD3'BD5’AOX1A-FACTORBAC1BAC2BGHrevCMV-FCMV-RDsRed1-C-rDsRed1-N-fEBVReverseEBV-RGAL4AD-FGAL4BDGLP1GLP2M13(-96M13FM13F（-47）M13RM13R（-48）P24990P31430P12584P17110PAS2-1FPAS2-1RPB42ADFPB42ADRpbd-gal4-fpbd-gal4-rpbi-121(35sPBV220FPBV220RPCDNA3.0-F2PCDNA3.0-R2PCDNA3.1F引物序列5'-GGCAAATGGCATTCTGACAT-3'5'-AGATGGTGCACGATGCACAG-3'5'-TAAGAGTCACTTTAAAATTTGTATC-3'5'-GACTGGTTCCAATTGACAAGC-3'5'-TACTATTGCCAGCATTGCTGC-3'5'-AACCATCTCGCAAATAAATA-3'5'-ACGCACAGAATCTAGCGCTT-3'5'-TAGAAGGCACAGTCGAGGC-3'5'-CGCAAATGGGCGGTAGGCGTG-3'5'-GTTCACGGTGCCCTCC-3'5'-AGCTGGACATCACCTCCCACAACG-3'5'-GTACTGGAACTGGGGGGACAG-3'5'-TGTGGTTTGTCCAAACTCAATC-3'5'-GTGGTTTGTCCAAACTCATC-3'5'-TACCACTACAATGGATG-3'5'-TCATCGGAAGAGAGTAG-3'5'-TGTATCTTATGGTACTGTAACTG-3'5'-CTTTATGTTTTTGGCGTCTTCCA-3'5'-CCCTCATAGTTAGCGTAACG-3'5'-TGTAAAACGACGGCCAGT-3'5'-CGCCAGGGTTTTCCCAGTCACGAC-3'5'-CAGGAAACAGCTATGACC-3'5'-AGCGGATAACAATTTCACACAGGA-3'5'-TCATCGGAAGCGAGTAG-3'5'-CGTTTTAAAACCTAAGAGTCAC-3'5'-TTTTCAGTATCTACGAT-3’5'-TACCACTACAATGGATG-3’5'-TCATCGGAAGCGAGTAG-3'5'-CGTTTTAAAACCTAAGAGTCAC-3'5'-CCAGCCTCTTGCTGAGTGGAGATG-3'5'-AAGCCGACAACCTTGATTGGAG-3'5'-GCCTCTAACATTGAGACAGC-3'5'-AAGAGTTACTCAAGAACAAGAA-3'5'-GACGCACAATCCCACTATCC-3'5'-AAGAAGGGCAGCATTCAAAG-3'5'-CTGCGTTCTGATTTAATCTG-3'5'-CTCTGGCTAACTAGAGAACC-3'5'-GGCAACTAGAAGGCACAGTC-3'5'-CTAGAGAACCCACTGCTTAC-3’
PCDNA3.1RpCEPFrorwardPCEP-FPCMV-FPCMV-FPCMV-RPCMV-RPDONR-FPDONR-RPEF-FPEGFP-C-3’PEGFP-C-5’PEGFP-N-3’PEGFP-N-5’pETupPGEX-3’PGEX-5’PIRES2-EGFP-FPIRES3-EGFP-RpMAL-C2X-FpMAL-C2X-RPQE30FPQE30RpTARGET-SEQPTRC99C-FPTRC99C-RPTRCHISA-FPTRCHISA-RRVP3RVP4S.TAGS1S6SP6T3T7T7（17BASE）T7TERTRXFTRXRmalEforpTrcHis-fwXpressfor5'-TAGAAGGCACAGTCGAGG-3’5'-AGCAGAGCTCGTTTAGTGAACCG-3'5'-AGAGCTCGTTTAGTGAACCG-3'5'-TCTAAAAGCTGCGGAATTGT-3'5'-TTCCAAAATGTCGTAATAAC-3'5'-TCCAAACTCATCAATGTATC-3'5'-ATTATAGAGGACACCTAGTC-3'5'-TCGCGTTAACGCTAGCATGGATCTC-3'5'-GTAACATCAGAGATTTTGAGACAC-3’5'-TCAAGCCTCAGACAGTGGTTC-3'5'-TATGGCTGATTATGATCAGT-3'5'-CATGGTCCTGCTGGAGTTCGTG-3'5'-CGTCGCCGTCCAGCTCGACCAG-3'5'-TGGGAGGTCTATATAAGCAGAG-3'5'-ACGATGCGTCCGGCGTAGAGGA-3'5'-CCGGGAGCTGCATGTGTCAGAGG-3'5'-GGGCTGGCAAGCCACGTTTGGTG-3'5'-GTAGGCGTGTACGGTGGGAG-3'5'-AACGCACACCGGCCTTATTC-3'5'-TGCGTACTGCGGTGATCAAC-3’5'-CTGCAAGGCGATTAAGTTGG-3’5'-TGAGCGGATAACAATTTCAC-3'5'-GTTCTGAGGTCATTACTGG-3'5'-TTACGCCAAGTTATTTAGGTGACA-3’5'-TTGCGCCGACATCATAAC-3’5'-CTGCGTTCTGATTTAATCTG-3’5'-CATGGTATGGCTAGCATGAC-3’5'-CAGGCTGAAAATCTTCTCTC-3’5'-CTAGCAAAATAGGCTGTCCC-3'5'-GACGATAGTCATGCCCCGCG-3'5'-CGAACGCCAGCACATGGACA-3'5'-CAACGTGAAAAAATTATTATTCGC-3’5'-GTAAATGAATTTTCTGTATGAGG-3’5'-ATTTAGGTGACACTATAG-3'5'-ATTAACCCTCACTAAAGGGA-3'5'-TAATACGACTCACTATAGGG-3'5'-ACATCCACTTTGCCTTTCTC-3'5'-TGCTAGTTATTGCTCAGCGG-3'5'-CATATGAGCGATAAAATTATTCAC-3'5'-GGATCCCTTGTCATCGTCATCACCA-3'5'-GGTCGTCAGACTGTCGATGAAGCC-3'5'-TTAAAGAGGTATATATTAATGTATCG-3'5'-AGCATGACTGGTGGACAG-3'

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